BD Difco™ Yeast Nitrogen w/o AA & AS 

Used in molecular genetics for classifying yeasts based on carbon and nitrogen requirements. 

BD™ Difco™ Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate is prepared according to the formulas of Wickerham.

Media with Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate are included in many applications for the study of yeasts in molecular genetics. Yeasts are unicellular, eukaryotic, budding cells that are generally round-to-oval or elongate in shape. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Yeasts are considered opportunistic pathogens.

• Contains all essential nutrients and vitamins necessary for the cultivation of yeasts and a source of nitrogen and carbohydrate

• Lacks the amino acids histidine, methionine and tryptophan and ammonium sulfate

• Prepared according to Guenter’s modification of Wickerham’s Yeast Nitrogen Base formulation

• Displays a good cultural response to Kloeckera apiculata 9774 and Saccharomyces cerevisiae 9080

• pH (0.17% solution, 25°C): 4.5 ± 0.2