BD Difco™ Bottle YPD Broth 500G 

Used for maintaining and propagating yeasts. BD™ Difco™ YPD Broth is a dehydrated powder 

for preparing culture media in molecular microbiology procedures.

General methods in yeast genetics specify using yeast extract-peptone-dextrose (YPD) medium for cultivating Saccharomyces 

cerevisiae and other yeasts. Yeasts grow well on a minimal medium containing only dextrose and salts. 

The addition of protein and yeast cell extract hydrolysates allows faster growth 

so that during exponential or log-phase growth, the cells divide every 90 minutes.

• Peptone acts as a source of carbon, nitrogen, vitamins and minerals

• Yeast extract supplies B-complex vitamins, which stimulate bacterial growth

• Dextrose is the carbohydrate source

• pH (6.5% solution, 25°C): 6.5 ± 0.2